Harmine inhibits COX-2 expression in gastric cancer cells through PTEN/Akt/MDM2 signaling pathway
10.3969/j.issn.1001-1978.2019.05.021
- Author:
Ting-Ting TAO
1
Author Information
1. Dept of Geriatric Gastroenterology, First Affiliated Hospital, Nanjing Medical University
- Publication Type:Journal Article
- Keywords:
Akt;
COX-2;
Gastric cancer;
Harmine;
MDM2;
PTEN
- From:
Chinese Pharmacological Bulletin
2019;35(5):695-700
- CountryChina
- Language:Chinese
-
Abstract:
Aim To further analyze the effects of PTEN/Akt/MDM2 signaling pathway in the harmine (HM)-mediated inhibition of COX-2 expression in gastric cancer cells. Methods PTEN-siRNA, Akt-siR-NA, MDM2-siRNA were constructed and respectively transfected into SGC-7901 and MKN45 cells,and then added or not added HM for 24 h. The expression of PTEN, Akt and phosphorylated Akt ( p-Akt), MDM2 and phosphorylated MDM2 ( p-MDM2), as well as COX-2 expression was detected by Western blot. Results HM increased PTEN expression, but inhibited p-Akt,p-MDM2 and COX-2 expression in SGC-7901 and MKN45 cells. Knockdown of PTEN blocked HM-induced inhibition of Akt and MDM2 phosphorylation, as well as down-regulation of COX-2 expression. Knockdown of Akt and treatment with HM synergisti-cally inhibited p-MDM2 and COX-2 expression. Knockdown of MDM2 and treatment with HM synergis-tically inhibited COX-2 protein expression. Conclusions HM down-regulates the expression of COX-2 protein in gastric cancer cells via PTEN/Akt/MDM2 signaling pathway.
