Propofol attenuates neuroinflammation and brain damage via modulating PI3K/Akt signaling pathway in rats of focal cerebral ischemia
10.3969/j.issn.1001-1978.2019.05.009
- Author:
Xian-Kun TU
1
Author Information
1. Dept of Neurosurgery, Fujian Medical University Union Hospital, Fujian Neurosurgery Institute
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
Inflammation;
LY294002;
PBK/Akt;
Propofol;
Signaling pathway
- From:
Chinese Pharmacological Bulletin
2019;35(5):630-633
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore whether propofol attenuates neuroinflammation and brain damage via modulating PI3K/Akt signaling pathway following focal cerebral ischemia in rats, and further investigate the possible mechanisms. Methods Sprague-Dawley rats which underwent the cerebral ischemic injury by the suture occlusion model were randomly divided into sham operation, MCAO, propofol-treated and LY294002 groups. Neurological deficit scores, cerebral infarct size, and cerebral water content were measured , then the myeloperoxidase (MPO) activities in rat brain were measured as an index of neutrophil infiltration. The content of TNF-α and IL-1β in blood was determined using ELISA; the expressions of p-Akt and Akt in rat brain were detected by Western blot. Results Propofol reduced neurological deficit scores, cerebral infarct size, cerebral water content, MPO activity , TNF-α and IL-1β content, which were all abolished by LY294002. Propofol up-regulated the expression of p-Akt, which was inhibited by LY294002. Conclusion Propofol attenuates neuroinflammation and ischemic brain damage via modulating the PI3K/Akt signaling pathway.
