Correlations between the CagA Antigen and Serum Levels of Anti-Helicobacter pylori IgG and IgA in Children.
10.3346/jkms.2016.31.3.417
- Author:
Ji Hyun SEO
1
;
Chun Woo LIM
;
Ji Sook PARK
;
Jung Sook YEOM
;
Jae Young LIM
;
Jin Su JUN
;
Hyang Ok WOO
;
Hee Shang YOUN
;
Seung Chul BAIK
;
Woo Kon LEE
;
Myung Je CHO
;
Kwang Ho RHEE
Author Information
1. Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea. hsyoun@gnu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Antibodies IgG/IgA;
Helicobacter pylori;
CagA Protein;
Urease;
Children
- MeSH:
Adolescent;
Antibodies, Bacterial/*blood;
Antigens, Bacterial/*analysis/immunology;
Bacterial Proteins/*analysis/immunology/metabolism;
Blotting, Western;
Child;
Child, Preschool;
Chronic Disease;
Enzyme-Linked Immunosorbent Assay;
Female;
Gastritis/pathology;
Helicobacter Infections/blood/microbiology/*pathology;
Helicobacter pylori/isolation & purification/*metabolism;
Humans;
Immunoglobulin A/*blood;
Immunoglobulin G/*blood;
Infant;
Infant, Newborn;
Male;
Severity of Illness Index;
Urease/metabolism
- From:Journal of Korean Medical Science
2016;31(3):417-422
- CountryRepublic of Korea
- Language:English
-
Abstract:
We tested correlations between anti-Helicobacter pylori IgG and IgA levels and the urease test, anti-CagA protein antibody, degree of gastritis, and age. In total, 509 children (0-15 years) were enrolled. Subjects were stratified as 0-4 years (n = 132), 5-9 years (n = 274), and 10-15 years (n = 103) and subjected to the urease test, histopathology, ELISA, and western blot using whole-cell lysates of H. pylori strain 51. The positivity rate in the urease test (P = 0.003), the degree of chronic gastritis (P = 0.021), and H. pylori infiltration (P < 0.001) increased with age. The median titer for anti-H. pylori IgG was 732.5 IU/mL at 0-4 years, 689.0 IU/mL at 5-9 years, and 966.0 IU/mL at 10-15 years (P < 0.001); the median titer for anti-H. pylori IgA was 61.0 IU/mL at 0-4 years, 63.5 IU/mL at 5-9 years, and 75.0 IU/mL at 10-15 years (P < 0.001). The CagA-positivity rate was 26.5% at 0-4 years, 36.5% at 5-9 years, and 46.6% at 10-15 years for IgG (P = 0.036), and 11.3% at 0-4 years, 18.6% at 5-9 years, and 23.3% at 10-15 years for IgA (P < 0.001). Anti-H. pylori IgG and IgA titers increased with the urease test grade, chronic gastritis degree, active gastritis, and H. pylori infiltration. Presence of CagA-positivity is well correlated with a high urease test grade and high anti-H. pylori IgG/IgA levels.
