Effect of rgs4 overexpression on related protein expression of mglur5-mediated signaling pathway in methamphetamine-dependent rat striatum
10.3969/j.issn.1001-1978.2019.08.016
- Author:
Lin-Bo ZHAO
1
Author Information
1. School of Institute of Information, Yunnan University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Cpp;
Gaq;
Methamphetamine;
Mglur5;
Plcß1;
Rgs4;
Striatum
- From:
Chinese Pharmacological Bulletin
2019;35(8):1115-1120
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of the regulator of G-protein signaling 4(RGS4) overexpression in rat striatum on the related protein expression of metabolic glutamate receptor 5(mGluR5) signaling pathway and the conditioned place preference(CPP) behavior in rats, by establishing the METH-dependent CPP model. Methods Rats were divided into five groups: Normal, normal saline(NS), METH, Ad5-RGS4-EGFP and Ad5-EGFP group. Normal group was without any administration, while the striatum of the other groups were respectively stereotactic injected with phosphate buffer methamphetamine (METH)-depardent soline (PBS), PBS, overexpressed adenovirus vector Ad5-RGS4-EGFP and negative control adenovirus vector Ad5-EGFP. The CPP behavior of rats in each group was analyzed. The expression of RGS4, mGluR5, Gαq, PLC1 was measured in rat striatum tissue by Western blot. Results The difference of CPP in Ad5-RGS4-EGFP group decreased compared with that in METH group and Ad5-EGFP group(P < 0.05), and increased compared with that in NS group(P < 0.05). RGS4 expression in Ad5-RGS4-EGFP group increased compared with that in other groups(P < 0.05, P < 0.01), the expression levels of mGluR5, Gαq decreased compared with those in METH group and Ad5-EGFP group(P < 0.05), while slightly increased compared with that in normal group and NS group(P>0.05). PLCβ1 expression changed with no significant difference. Conclusions RGS4 overexpression in striatum is able to alleviate the CPP behavior in METH-dependent rats, and its mechanism may be associated with the overexpression of RGS4 in METH-dependent rat striatum, which could down-regulate the mGluR5-mediated Gαq and PLCβ1 signaling pathway.
