Chemical constituents of Pteridium aquilinum supercritical extract and its antiadipogenic effect on 3T3L1 preadipocytes
10.3969/j.issn.1001-1978.2019.11.020
- Author:
Yongxiang WU
1
Author Information
1. College of Life and Environment Science, Huangshan University
- Publication Type:Journal Article
- Keywords:
3T3-L1 cells;
Adipocyte differentiation;
Chemical constituents;
Molecular mechanism;
Pteridium aquilinum;
Supercritical C02 extraction
- From:
Chinese Pharmacological Bulletin
2019;35(11):1590-1595
- CountryChina
- Language:Chinese
-
Abstract:
Aim To identify chemical constituents of supercritical extract from Pteridium aquilinum (Linn.) Kuhn (SFEPA) and investigate its antiadipogenic effect and underlying mechanism. Methods The chemical constituents of SFEPA were identified by GCMS. The differentiation of 3T3L1 preadipocytes was induced to establish cell model. MTT assay was employed to detect cell viability. Oil Red 0 staining was used to analyze the effect of SFEPA on the differentiation of 3T3L1 preadipocytes. qRTPCR was performed to examine the expression of genes related with adipogenesis and lipogenesis. Results Ten compounds of SFEPA were identified. The major constituents were 4, 4, 5, 7, 8pentamethyldihydrocoumarin (36. 0 7 %), ßsitosterol (25. 6 6 %), 4amino7diethylaminochromen2one (8 . 2 6 %) and palmitic acid (5. 0 5 %). SFEPA suppressed 3T3L1 adipocyte differentiation and reduced fat accumulation in a dosedependent manner. Treatment of SFEPA significantly downregulated the mRNA expression of PPARy, C/ EBPa, SREBPlc, as well as FAS and ACC. Conclusions SFEPA can inhibit the adipogenesis and differentiation of 3T3L1 cells, and its mechanism may be closed related to its inhibition of genes expression regulating adipogenesis and lipogenesis. Phenols and alcohols might be potential bioactive components.
