Biological features of tumor associated fibroblasts and effect of Transwell co-culture on proliferation and metastasis of gastric cancer cells
10.3969/j.issn.1001-1978.2019.11.019
- Author:
Jiu-zhen SHAO
1
Author Information
1. Pharmacology and Toxicology Lab of Traditional Chinese Medicine, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine in Nanjing University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Cancer associated fibroblasts;
Gastric cancer;
Heterogeneity;
Primary culture;
The reverse Warburg effect;
Transwell co-culture
- From:
Chinese Pharmacological Bulletin
2019;35(11):1582-1589
- CountryChina
- Language:Chinese
-
Abstract:
; Aim To culture primary human gastric cancer associated fibroblasts (CAFs) and normal fibroblasts (NFs), and to explore the biological characteristics and their effects on gastric cancer cells. Methods After isolation and culture of CAFs and NFs, growth curve was drawn by MTT. The a-smooth muscle actin (ot-SMA) and Vimentin were detected by Immunofluorescence, Western blot and qRT-PCR. MGC-803 cells were co-cultured with CAFs and NFs in Transwell suspension mode. The migration and invasion ability of gastric cancer cells was detected by Transwell. The proliferation activity and AMD3100 on CAFs-gastric cancer co-culture system were compared by MTT. The acidic property, lactic acid and ROS contents of co-culture system were determined by PH meter, lactic acid and DCFH-DA method. Results The morphology of CAFs, NFs cells were in long spindle or flat star shape. The proliferation ability and overlapping growth phenomenon of CAFs were higher than those of NFs. The expression of ct-SMA and Vimentin cells was positive in CAFs, but low or negative in NFs cells. The activity of gastric cancer in low density co-culture group > medium density group > high density group, the PH value of CAFs co-culture system decreased, the content of lactic acid and ROS was high, and only CAFs low density co-culture group had significant effect on promoting cancer. Conclusions The co-culture of gastric cancer cells with CAFs and NFs is greatly affected by the proportion. Low density co-culture can significantly improve the proliferation and metastasis ability of gastric cancer cells. High density co-culture may in turn inhibit the growth and metastasis of cancer cells, which may be related to the content of lactic acid and ROS.
