Chaiqinchengqi decoction alleviates liver injury during severe acute pancreatitis in mice via inhibition of tlr4/nf-kb p65 pathway
10.3969/j.issn.1001-1978.2020.01.026
- Author:
Lin DANG
1
Author Information
1. College of Basic Medicine
- Publication Type:Journal Article
- Keywords:
Chaiqinchengqi decoction;
Endotoxin;
Liver injury \;
Nf-kb;
Severe acute pancreatitis;
Tlr4
- From:
Chinese Pharmacological Bulletin
2020;36(1):134-139
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the protective mechanisms of Chaiqinchengqi decoction (CQCQD) on liver injury during severe acute pancreatitis ( SAP) based on TLR4/NF-kB p65 pathway. Methods Thirty-six KM mice were randomly divided into control group (Control) , SAP and SAP + CQCQD (treatment group) (n = 12). The mice in SAP group were injected with 20% L-arginine intraperitoneal^ (3.3 g • kg"1, 2 times, 1 h interval). The mice in SAP + CQCQD group were intragastrically administered with Chaiqinchengqi decoction (19 g • kg-1 • d"1) following induction of SAP. Histopathologic^ changes of pancreas and liver were observed 72 hours after model establishment. The endotoxin in serum was tested, and the TLR4 and p-NF-kb p65 expression, as well as some inflammatory cytokines in liver was detected. Re-suits Compared to control group, the pathological damages of the pancreas and liver in SAP group were aggravated, serum endotoxin increased, the expression of TLR4 and p-NF-kB p65 in liver increased, and IL-6, TNF-a and MIP-1 a mRNA in liver were significantly elevated. Compared to SAP group, the intervention of CQCQD partially relieved the pathological damages of the pancreas and liver, reduced the endotoxin in serum , and decreased the expression of TLR4 and p-NF-kB p65, as well as IL-6, TNF-a and MIP-1 a mRNA in the liver. Conclusion CQCQD appears to protect liver injury during SAP via inhibiting TLR4/NF-kB p65 activation, then decreasing cytokines and chemo-kines.
