Berberine inhibits tgf-pl-induced epithelial-mesenchymal transition in human liver cancer hepg2 cells via tgf-fi/smad pathway
10.3969/j.issn.1001-1978.2020.02.021
- Author:
Chun-Miao CHEN
1
Author Information
1. College of Pharmacy
- Publication Type:Journal Article
- Keywords:
Berberine;
Cell invasion;
Cell migration;
Epithelial-mesenchymal transition (emt);
Human liver cancer hepg2 cells;
Tgf-p/smad signaling pathway;
Tgf-pl
- From:
Chinese Pharmacological Bulletin
2020;36(2):260-266
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of berberine on epithelial-mesenchymal transition (EMT) of human liver cancer HepG2 cells induced by transforming growth factor-pi ( TGF-pl ) and its mechanism. Methods MTT assay was used to detect the proliferation activity of berberine on HepG2 cells. After 10 ng • L"1 TGF-pl was used to induce EMT model process of HepG2 cells, berberine was added to treat HepG2 cells. Colony formation, cell scratch and Transwell assays were used to detect the clonogenic, migratory and invasive capabilities of HepG2 cells. Immunofluorescence assay was used to detect the expression of EMT mesenchymal marker Vimentin. Western blot assay was used to detect the proteins expression of EMT marker (E-cadherin, N-cadherin, Snail), matrix metallopro-teinase ( MMP-2), TGF-p/Smad pathway (Smad2, p-Smad2, Smad3, p-Smad3) in HepG2 cells. Results Berberine inhibited the proliferation of HepG2 cells in a concentration-time dependent manner. Compared with TGF-pl group, berberine could significantly inhibit the abilities of colony formation, migration and invasion of HepG2 cells. Berberine could significandy inhibit the expression of E-cadherin protein up-regula-ted by TGF-pl, and N-cadherin, Vimentin;, Snail, MMP-2, p-Smad2, p-Smad2 proteins expression down-regulated by TGF-pl. Conclusions Berberine may interfere with the EMT process of HepG2 cells induced by TGF-pl by inhibiting the TGF-p/Smad 'signaling pathway to inhibit the HepG2 cell migration and invasion.
