Mechanism study of resveratrol decreasing expression of gdf8 via autophagy in skeletal muscle of obese mice
10.3969/j.issn.1001-1978.2020.02.015
- Author:
Yun-Fei FEI
1
Author Information
1. Anhui Medical University, Dept of Biochemistry and Molecular Biology
- Publication Type:Journal Article
- Keywords:
Autophagy;
Gdf8;
Obesity;
Resveratrol;
Sirt1;
Skeletal muscle
- From:
Chinese Pharmacological Bulletin
2020;36(2):226-231
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the mechanism of resveratrol decreasing GDF8 expression via activating autophagy in skeletal muscle of obese mice. Methods Male C57BL/6 mice were randomly divided into standard control diet (SCD), SCD treated with resveratrol (SCD + RES), high-fat diet (HFD) and high-fat diet group treated with resveratrol (HFD + RES). The SCD + RES and HFD + RES groups were intervened with resveratrol via gavage daily (400 mg • kg"1 • d-1) for 20 weeks. The body mass, serum TG, TC, LDL-C and HDL-C levels were detected. The pathological changes in skeletal muscle were detected by HE staining. The mRNA, protein distribution and expression of SIRT1, LC3, p62 and GDF8 were assessed by im-munohistochemistry, RT-PCR and Western blot respectively. Results The body mass , serum TG , TC and LDL-C levels increased, while HDL-C levels decreased in HFD group. Lipid deposition between skeletal muscle fibers was obvious in HFD group. The mRNA and protein expressions of GDF8 and p62 increased, while the mRNA and protein expressions of S1RT1 and LC3 decreased. All the response was attenuated by treatment with resveratrol. Conclusions High-fat diet in-\ duces lipid depostion and decreases autophagy in skeletal muscle. Resveratrol treatment significantly attenuates GDF8 expression in skeletal muscle tissues, which may be related with activated SIRTland induced autophagy, thus resulted in the degradation of myostatin.
