Sal b attenuates cardiomyocyte injury by regulating priming of nlrp3 inflammasomes
10.3969/j.issn.1001-1978.2020.02.012
- Author:
Yang HU
1
Author Information
1. College of Pharmacy
- Publication Type:Journal Article
- Keywords:
Hypoxia;
Myocardial cell injury;
Nf-kb;
Nl-rp3;
Priming;
Salvianolic acid b
- From:
Chinese Pharmacological Bulletin
2020;36(2):210-215
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate whether Sal B alleviates hypoxic-induced cardiomyocyte injury by regulating the priming of NLRP3 inflammasomes. Methods The effects of Sal B on growth of H9C2 cells were examined by CCK8 assay,then the appropriate concen tration of Sal B was selected. The expression level of LDH was detected by Microliter assay. The expression levels of cTn/IL-lp were measured by Elisa assay. The protein and mRNA levels of TLR4/Myd88/I-RAK1/NF-kB/NLRP3 were detected by Western blot and qPCR. Results Hypoxia intervention notably reduced the viability of H9C2 cells and increased the expression levels of cTn/IL-IP. Besides,the protein and mRNA expression levels of TLR4/Myd88/IRAK1/NF-kB/NLRP3 were significant uP-regulated after hypoxia intervention. However, the viability of H9C2 cells increased, the secretion levels of LDH/cTn/IL-1 p were reduced,and the protein and mRNA levels of TLR4/Myd88/IRAK1/NF-KB/NLRP3 were significant inhibited after pretreated with Sal B. Conclusion Sal B attenuates cardiomyocyte injury by regulating the priming of NLRP3 inflammasome.I.
