Research on anti-non-small cell lung cancer activity of ornithine decarboxylase anti-enzyme inhibitor-1 small molecule inhibitor
- Author:
Shun-Chao WANG
1
Author Information
1. Dept of Immunology, Medical College, China Three Gorges University
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cell proliferation;
Non-small cell lung cancer;
Ornithine decarboxylase anti-enzyme inhibitor-1;
Polyainines;
Small molecule inhibitor
- From:
Chinese Pharmacological Bulletin
2020;36(4):556-561
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the inhibitory effect of small molecule inhibitors of ornithine decarboxylase inhibitor 1 (AZIN-1) on non-small cell lung cancer and its mechanism. Methods Cell proliferation was detected by Cell Counting Kit-8 (CCK-8). Apoptosis was analyzed by flow cytometry (PI/Annexin V-FITC double staining). The expression of ornithine decarboxylase (ODC), ornithine decarboxylase anti-enzyme-1 (AZ-1) and AZIN-1 was detected by Western blot. Cell cycle was analyzed by flow cytometry (PI single staining). The total polyamine content in cellswas measured by high performance liquid chromatography (HPLC). Results Small molecule inhibitor of AZIN-1 could significantly inhibit the proliferation of A549 cells, cause G0/G, cycle arrest, induce apoptosis of A549 cells, inhibit the expression of AZIN-1 and ODC, interfere with intracellular polyamine metabolism, and reduce total polyamine content in cells. Conclusions Small molecule inhibitor of AZIN-1 has significant growth inhibitory effect on A549 cells, and its mechanism may be related to the induction of apoptosis and interference with polyamine metabolism.
