Enhancive effect of FGL1 gene silencing on sensitivity of human lung adenocarcinoma PC-9 cells to docetaxel
10.3969/j.issn.1001-1978.2020.05.019
- Author:
Ji-Qing HAO
1
Author Information
1. Dept of Oncology, First Affiliated Hospital of Anhui Medical University
- Publication Type:Journal Article
- Keywords:
Chemotherapy;
Docetaxel;
Fibroblast-like protein 1;
Lung adenocarcinoma;
PC-9 cells;
Proliferation
- From:
Chinese Pharmacological Bulletin
2020;36(5):687-691
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of fibrinogenlike protein 1 (FGL1)) silencing on docetaxel sensitivity in human lung adenocarcinoma PC-9 cells. Methods Western blot was used to detect the expression of FGL1 protein in human normal bronchial epithelial cell line BEAS-2B and human lung adenocarcinoma cell line PC-9. The FGL1 gene in PC-9 cell line was silenced by siRNA. CCK-8 assay was used to detect the inhibitory effect of silencing FGL1 on PC-9 cell proliferation and its effect on docetaxel sensitivity. Results Compared with BEAS-2B cell line, FGL1 was highly expressed in PC-9 cell line, and the relative expression of FGL1 protein was 6. 5 times that of BEAS-2B cell line with statistically significant difference (P <0. 01). Silencing FGL1 by transfection with FGLlsiRNA could enhance the inhibitory effect of docetaxel on PC-9 cells. Compared with FGLlsiNC group, the IC50value of PC-9 cells in FGL1siRNA group was significantly reduced with statistically significant difference (P < 0. 01). Conclusions Specific silencing of FGL1 gene could inhibit the expression of FGL1 in human lung adenocarcinoma cell PC-9, inhibit the proliferation of PC-9 cells and increase the sensitivity to docetaxel.
