An experimental study of SCAD recombinant adenovirus treating heart failure following myocardial infarction in rats
10.3969/j.issn.1001-1978.2020.05.005
- Author:
Yong-Shao SU
1
Author Information
1. Dept of Clinical Pharmacy, School of Pharmaceutical Sciences, Guangdong Pharmaceutical University
- Publication Type:Journal Article
- Keywords:
Energy metabolism;
Fatty acid beta oxidation;
Heart failure;
Mitochondria;
Recombinant adenovirus;
Short-chain acyl-CoA dehydrogenase
- From:
Chinese Pharmacological Bulletin
2020;36(5):608-615
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of overexpression of short-chain acyl-CoA dehydrogenase (SCAD) recombinant adenovirus on heart failure following myocardial infarction in rats, and to explore the relationship between SCAD and heart failure. Methods The rat model of heart failure following myocardial infarction was established by ligation of left anterior descending coronary artery (LAD). The SCAD recombinant adenovirus was injected into the apical wall. The experimental groups were divided into 6 groups; Sham + NS group, LAD + NS group, Sham + Ad-GFP group, Sham + Ad-SCAD group, LAD + Ad-GFP group and LAD + Ad-SCAD group. Systolic blood pressure (SBP) of tail artery was measured after surgery and the cardiac morphological changes were detected. The changes of SCAD mRNA expression, protein expression, enzyme activity, ATP content and free fatty acid content were examined. Results Compared with sham group, LAD + NS group showed heart failure significantly. Compared with LAD + NS group, SBP from LAD + Ad-SCAD group increased obviously after surgery, collagen deposition was apparently reduced and myocardial fibrosis was markedly improved. Compared with Sham + NS group, the SCAD mRNA, protein expression, enzyme activity and ATP content in LAD + NS group obviously decreased, and the contents of free fatty acids markedly increased. Compared with LAD + NS group, the above indicators of LAD + Ad-SCAD group were evidently reversed after treated with SCAD recombinant adenovirus. Conclusion Overexpression of SCAD recombinant adenovirus in heart can significantly improve heart failure caused by LAD.
