Role of NR2B and CREB in ginsenoside Rbl regulation of methamphetamine-induced conditioned place preference in rats
10.3969/j.issn.1001-1978.2020.05.004
- Author:
Gen-Meng YANG
1
Author Information
1. School of Forensic Medicine, Kunming Medical University
- Publication Type:Journal Article
- Keywords:
Conditioned place preference;
CREB;
Ginsenoside rb1;
Methamphetamine;
Neurotoxicity;
NR2B
- From:
Chinese Pharmacological Bulletin
2020;36(5):604-608
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of ginsenoside Rbl on methamphetamine-induced CPP in rats and to explore the role of NR2B/CREB in it. Methods METH(2mg·kg-1, i.p) was administered to establish METH-induced CPP model in rats. 0 ∼3 d was the adaptation stage and 4 ∼ 13 d was the experimental stage. METH (2 mg · kg-1, i. p) or saline (10 mg · kg-1, i. p) was injected every other day. Rb1 (10 mg · kg-1, i.p) or saline was pre-injected lh before injection of METH or saline. After perfusion, the hippocampus was isolated from brain on ice, and the expression levels of NR2B, CREB and p-CREB were detected by Western blot. Results The animal model of METH-induced CPP was successfully established. The rats were pretreated with Rbl (10 mg · kg-1) for 1 h, and the time that the rats stayed in drug-paired was significantly reduced compared with METH group. Western blot results showed that NR2B, p-CREB and p-CREB/CREB significantly increased in METH group and without altering CREB expression levels compared with control group. However, after pre-treated with Rbl, the expression levels of NR2B, p-CREB and p-CREB/CREB decreased compared with METH group. Conclusions METH can significantly induce CPP in rats. Rbl may inhibit METH-induced CPP in rats by regulating NR2B and p-CREB.
