ESTABLISHING AN ANIMAL MODEL OF DEFECATION RECONSTRUCTION AFTER SPINAL CORD INJURY IN RATS BY MECHANICAL POLISHING METHOD
10.7507/1002-1892.20160232
- Author:
Bingbo BAO
1
Author Information
1. Department of Orthopedic Surgery, Shanghai JiaoTong University Affiliated Sixth People's Hospital
- Publication Type:Journal Article
- Keywords:
Animal model;
Bites method;
Defecation reconstruction;
Mechanical polishing method;
Rat;
Spinal cord injury
- From:
Chinese Journal of Reparative and Reconstructive Surgery
2016;30(9):1139-1142
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To study the feasibility and advantages of preparing an animal model of defecation reconstruction after spinal cord injury in rats by mechanical polishing method. METHODS: Forty adult female Sprague Dawley rats (weighing, 250-300 g) were randomly divided into 2 groups (n=20). The lamina was opened by mechanical polishing method to expose the cauda equina in experimental group, then bilateral L5 and S1 nerve roots end-to-end anastomosis was done under 10 times microscope, and finally cauda equina between the L5 and L6 (except S1) was cut. The lamina was opened by traditional bites method in control group, and the other treatment methods were in agreement with the experimental group. The operative time, intra-operative blood loss, and situation of rats at postoperative 3 days were recorded. RESULTS: The operative time of experimental group[(93.05±7.60) minutes] was significantly shorter than that in control group[(131.30±11.68) minutes] (t=12.279, P=0.000); intra-operative blood loss in experimental group[(4.33±0.46) mL] was significantly lower than that in control group[(7.36±0.58) mL] (t=18.293, P=0.000). At 3 days after operation, 18 rats (90%) survived in experimental group, and 12 rats (60%) survived in control group; difference was significant in the survival rate between 2 groups (χ2=4.800, P=0.028). CONCLUSIONS: To establish an animal model of defecation reconstruction after spinal cord injury in rats by mechanical polishing method is feasible, and it has shorter operative time, less blood loss, and lower postoperative mortality than the traditional bites method. But there is a certain learning curve and requirement to master microsurgical techniques.
