Effect of FTY720-P on the differentiation and maturation of MC3T3-E1 cells
10.7507/1002-1892.201710108
- Author:
Libin ZHENG
1
Author Information
1. Guangdong Medical University, Zhanjiang Guangdong
- Publication Type:Journal Article
- Keywords:
cell apoptosis;
FTY720-P;
MC3T3-E1 cells;
osteoblasts
- From:
Chinese Journal of Reparative and Reconstructive Surgery
2018;32(3):285-290
- CountryChina
- Language:Chinese
-
Abstract:
Methods: The MC3T3-E1 cells were divided into the experimental group and the control group. In the experimental group, the cells were induced by the medium containing 400 ng/mL FTY720-P (chloroform as solubilizer) in vitro. In the control group, the cells were cultured with the medium only containing chloroform. The cell morphology of 2 groups were observed by inverted phase contrast microscope; the expression of osteoblast related protein (collagen type Ⅰ and collagen type Ⅲ) was detected by immunofluorescence staining; the alkaline phosphatase (ALP) staining and alizarin red staining were used to observe the formation of osteoblasts and the formation of mineralized nodules in 2 groups; and the TUNEL fluorescence assay was used to detect the cell apoptosis.
