The mechanism of neuroprotective effect of aspirin after cerebral ischemia/reperfusion injury in rats
- Author:
Tao XU
1
Author Information
1. Department of Neurology
- Publication Type:Journal Article
- From:
Chinese Journal of Cerebrovascular Diseases
2006;3(6):267-272
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the mechanism of neuroprotective effects of aspirin after cerebral ischemia/reperfusion injury in rats. Methods: Cerebral ischemia/reperfusion model of middle cerebral artery occlusion was established in rats using the suture method. Forty-eight experimental rats were divided into control group (group A), low-dose group (group B; aspirin 20 mg/kg), moderate-dose group (Group C; aspirin 80 mg/kg), and high-dose group (group D; aspirin 320 mg/kg), each group had 12 rats. Aspirin were continuously injected intraperitoneally in the rats in experimental groups for 3 days as an interference factor after cerebral ischemia/reperfusion. At the same day after cerebral ischemia/ reperfusion, the neurological function of all the rats in each group was evaluated for 4 days by the Bederson score and was documented. All the rats in each group were killed at day 4. Cerebral infarct volume were measured by TTC stain, the apoptosis in situ labeling were detected by TUNEL, and the expressions of apoptosis-regulating proteins BCL-2 and BAX were detected by inummohistochemistry. Results: Limb function improved in the groups of A, B, and C after aspirin intervention. As compared with the group A, the neurological deficit scores decreased significantly, and the infarct volume reduced significantly (compared with the group A, the infarct volume decreased 16.3%, 19.2%, and 12.8%, respectively); the positive rate of apoptosis cells in the surrounding ischemic areas decreased, they were 58%, 37%, 35%, and 40% in groups A, B, C, and D; the protein Bcl-2 expression in the ischemic areas increased 38%, 55%, 60%, and 50% in groups A, B, C, and D; and protein Bax expression decreased 50%, 34%, 33%, and 42% in groups A, B, C, and D. Among the 3 treatment groups, the efficacy of the low-and moderate-dose groups was better than that of the high-dose group. Conclusion: Early use of aspirin may decrease neurological defic its after cerebral ischemia/reperfusion in rats, and thus exert its neuroprotective effect.
