The relationship between protein kinase C isoenzyme, intracellular free calcium and apoptosis after intracerebral hemorrhage in rats
- Author:
Ling LI
1
Author Information
1. Department of Neurology
- Publication Type:Journal Article
- From:
Chinese Journal of Cerebrovascular Diseases
2006;3(10):440-444
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the relationship between protein kinase C (PKC) isoenzyme (PKCδ, PKCγ), intracellular free calcium concentration and apoptosis of peri-hematoma neurons at different time points after intracerebral hemorrhage (ICH) in rats and their significance. Methods: Thirty-six healthy adult Wistar rats were randomized into normal control group and 0.5, 6, 24, 72, 120 hours groups after ICH (n = 6 in each group). A rat model of ICH was induced by collagenase method. The expression of PKC isoenzyme was observed by immunohistochemistry; the concentration of intracellular free calcium was determined by the Fura-2/AM fluorescent probe, and apoptosis was detected with the TUNEL method. Results: The expression of PKCδ, PKCγ and the intracellular free calcium concentration were positively correlated with the numbers of apoptotic neurons around hematomas after ICH (r = 0.970, 0.984, respectively, P < 0.01). They began to increase at 0.5 hour after ICH, reached their peaks from 6 to 24 hours; and began to decrease at 72 hours, and decreased significantly at 120 hours. Conclusions: The expression of PKCδ, PKCγ upregulation and intracellular calcium overload around hematomas after ICH are closely associated with apoptosis. It is suggested that abnormal cell signal transduction may participate in physiopathological processes of neuronal injury after ICH.
