Expression changes of microRNA-124a in brain tissues and plasma in rats with cerebral ischemia
10.3969/j.issn.1672-5921.2011.03.007
- Author:
Yang LIN
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
Brain;
Brain ischemia;
MicroRNAs;
Plasma;
Rats
- From:
Chinese Journal of Cerebrovascular Diseases
2011;8(3):143-147
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression changes of microRNA-124a in plasma and brain tissues in rats with cerebral ischemia and its distribution in different tissues and different cells in rats. Methods: Thirty-five healthy male SD rats were randomized into 2 groups, the sham operation group and the model group (n = 16 in each group), 8 rats in each group were used to detect the expression of plasma microRNA-124a at before the modeling and at 2, 4, 8, 12, 24, and 48 hours after the modeling. Eight rats were used to detect the expression of plasma microRNA-124a at 24 hours in ischemic brain tissue; Three rats were used to detect the expression of microRNA-124a in different tissues in normal rats. A model of permanent middle cerebral artery occlusion was induced by the intraluminal suture method. Real-time quantitative PCR assay and reverse transcription polymerase chain reaction (RT-PCR) were used to detect the expression of microRNA-124a. Results: Circled digit onehe expression levels of microRNA-124a in brain, aorta, lung, liver, heart, spleen, muscle and kidney in rats were decreased successively, and the expression level in brain was 1116.68 (451.94-2740.08) times of the kidney. The expression levels of microRNA-124a in hippocampal neurons and microglia cells in rats were 1031.12 (501.50-2120.20) and 19.43 (13.20 to 28.60) times those in brain microvascular endothelial cells in mice. Circled digit twoCompared to before modeling, the expression level of microRNA-124a in plasma began to increase at 8 hours after modeling (P = 0.02). It reached the peak at 24 hours (P = 0.008), and it was about 18 times before modeling. Then it decreased gradually, but the expression level at 48 hours was still higher than that at 0 hour before modeling (P = 0.03). There were no significant differences in the expression of microRNA-124 at different time points before and after modeling in the sham operation group. Circled digit threeThe expression level of microRNA-124 in ischemic brain tissue in the model group was decreased significantly at 24 hours after modeling. It was about 12% to 28% of the sham operation group. Conclusion: microRNA-124a specifically expressed in brain neurons in rats. The expression level of microRNA-124a in plasma was increased significantly at 24 hours after cerebral ischemia, while the expression level in brain tissue was decreased significantly. The mechanism may be associated with the brain damage caused a large quantity of microRNA-124a releasing into blood.
