Lidocaine inhibits the malignant proliferation, invasion and regulates the mitochondrial respiration of osteosarcoma MG-63 cells
10.12092/j.issn.1009-2501.2020.05.006
- VernacularTitle: 利多卡因对骨肉瘤细胞MG-63恶性增殖,侵袭及线粒体呼吸作用的调节
- Author:
Yi CHEN
1
Author Information
1. Department of Anesthesiology, The First People's Hospital of Jingzhou (the First Affiliated Hospital of Changjiang University)
- Publication Type:Journal Article
- Keywords:
Lidocaine;
Malignant biological behavior;
Mitochondrial respiration;
Osteosarcoma
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2020;25(5):519-526
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of lidocaine on malignant proliferation, invasion and mitochondrial respiration of osteosarcoma MG-63 cells. METHODS: MG-63 cells were treated with 25, 50 and 100 μmol/L of lidocaine and were randomly divided into four groups: lidocaine 0 μmol/L, lidocaine 25 μmol/L, lidocaine 50 μmol/L and lidocaine 100 μmol/L for subsequent experiments. BrdU staining was used to detect cell proliferation. Transwell for cell invasion. Protein expression levels of Ki67, Survivin, VEGF and Vimentin were detected by Western blot. Mitochondrial membrane potential was detected by flow separator. Activity of mitochondrial respiratory complex was detected by Clark oxygen electrode method. The kit detected the content of ATP, SOD and MDA.RESULTS: Results showed that compared with lidocaine 0 μmol/L group, BrdU positive cells in lidocaine 50, 100 μmol/L group was significantly reduced (P<0.05), invasive cells was significantly reduced (P<0.05), Ki67, Survivin, VEGF, Vimentin protein levels decreased significantly (P<0.05), mitochondrial membrane potential decreased significantly, compound I, II, IV activity decreased significantly (P<0.05), ATP, SOD content decreased significantly (P<0.05), MDA content was significantly increased (P<0.05). CONCLUSION: Lidocaine can inhibit the malignant proliferation, invasion and improve the mitochondrial function of osteosarcoma MG-63 cells.
