Protective effects of Xiaochaihu Decoction on chemical hepatic fibrosis in mice
10.12092/j.issn.1009-2501.2020.05.001
- VernacularTitle: 小柴胡汤对化学性肝纤维化小鼠的保护作用
- Author:
Furong WU
1
Author Information
1. Department of Pharmacy, The First Affiliated Hospital of USTC
- Publication Type:Journal Article
- Keywords:
Carbon tetrachloride;
Hepatic fibrosis;
Mice;
Xiaochaihu Decoction
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2020;25(5):481-488
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the protective effects of Xiaochaihu Decoction (XCHD) on carbon tetrachloride (CCl4)-induced hepatic fibrosis (HF) in mice. METHODS: HF was induced in male mice by 20% CCl4 twice a week for 12 weeks. XCHD (3.90, 7.80, 15.60 g/kg) and Silybin (0.1 g/kg) were administrated via gavage once a day starting from the CCl4 treatment for subsequent 12 weeks. Then, the levels ALT and AST in serum were assayed, liver samples were taken to examine the degree of HF by HE, Masson, Sirius Red staining and Electron microscope. Moreover, the protein and mRNA expression levels of α-smooth muscle actin (α-SMA) and Collagen Ι were determined by RT-qPCR, immunofluorescence and Western blot. RESULTS: The data demonstrated that XCHD (7.80, 15.60 g/kg) effectively reduced histopathological changes, such as steatosis, collagen deposition; meanwhile the histopathological analysis suggested that XCHD obviously alleviated the degree of HF. Furthermore, XCHD significantly reduced the levels of ALT, AST (P<0.05), and attenuated the expressions of α-SMA, Collagen Ι both of protein and mRNA levels (P<0.05). CONCLUSION: XCHD has protective effect on chemical HF in mice.
