Effects of knocking down long intergenic non-coding RNA 00467 on the prognosis of patients with lung adenocarcinoma and its mechanism
10.12092/j.issn.1009-2501.2020.08.001
- VernacularTitle: 敲降长链非编码RNA 00467表达对肺腺癌患者预后的影响及其机制
- Author:
Xianghai WANG
1
Author Information
1. Department of Respiratory Medicine, Yijishan Hospital of Wannan Medical College
- Publication Type:Journal Article
- Keywords:
Linc00467;
Long non-coding RNA;
Lung adenocarcinoma;
Prognosis;
Proliferation
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2020;25(8):841-849
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effect of knocking down long intergenic non-coding RNA 00467 on the prognosis of patients with lung adenocarcinoma and its mechanism. METHODS: Quantitative reverse transcription PCR (qRT-PCR) was used to detect the expression level of linc00467 in the plasma of LAD patients and healthy volunteers. The overall survival (OS) was analyzed by Kaplan-Meier survival analysis and log-rank tests. Cell proliferation assays and Xenograft mouse model were used to confirm the effect of linc00467 expression on tumorigenesis in vitro and in vivo.RESULTS: linc00467 expression was up-regulated in the plasma of LAD patients compared with healthy volunteers. In addition, high levels of linc00467 expression were correlated with larger tumour sizes, lymph node metastasis and advanced TNM stages. High levels of linc00467 indicated a poor prognosis in LAD patients, multivariate analyses indicated that linc00467 expression could serve as an independent prognostic factor for overall survival of LAD. Functional experiments showed that knockdown of linc00467 could inhibit LAD cell proliferation in vitro and in vivo. CONCLUSION: linc00467 is involved in the progression of LAD and that linc00467 may be a novel diagnosis biomarker and a potential therapeutic target for LAD.
