Resveratrol regulates miR-409-3p/FKBP14 to inhibit the malignant biological processes of gastric cancer BGC823 cells
10.12092/j.issn.1009-2501.2020.09.002
- VernacularTitle: 白藜芦醇调控miR-409-3p/FKBP14抑制胃癌细胞BGC823恶性进程
- Author:
Yi WU
1
Author Information
1. Hangzhou First People's Hospital, Gastrointestinal and Anal Surgery
- Publication Type:Journal Article
- Keywords:
Gastric cancer;
MiR-409-3p;
Resveratrol
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2020;25(9):968-975
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of resveratrol (RES) on the malignant biological processes of gastric cancer (GC) cells. METHODS: CCK-8 assay was used to study the effect of RES on the viability of BGC823 cells; qRT-PCR was used to detect the regulation of RES on the expression of miR-409-3p; EdU assay was used to study the effect of RES on the proliferation of BGC823 cells; transwell assay was used to study the effect of RES on the migration and invasion of BGC823 cells; luciferase reporter assay and western blot assay were used to study the regulation of miR-409-3p on the expression of FKBP14. RESULTS: RES (>200 μmol/L) significantly inhibited the viability of BGC823 cells. RES inhibited the proliferation, migration and invasion of gastric cancer cells by up-regulating miR-409-3p. FKBP14 was the target gene of miR-409-3p. Res could inhibit the expression of FKBP14 in gastric cancer cells by up-regulating miR-409-3p. Overexpression of FKBP14 attenuated the antitumor effect of RES. CONCLUSION: RES can suppress the tumor progression in gastric cancer by regulating miR-409-3p/FKBP14 signaling pathway, indicating the potential value of RES in clinical treatment of gastric cancer.
