HPLC fingerprint on wild germplasm resource of Ophiopogon japonicus in Sichuan and its chemical pattern recognition
- Author:
Jiang LIU
1
Author Information
1. College of Agronomy
- Publication Type:Journal Article
- Keywords:
Fingerprint;
Ophiopogon japonicus (Thunb.) Ker-Gawl. in Sichuan;
Principal component analysis (PCA) clustering analysis
- From:
Chinese Traditional and Herbal Drugs
2010;41(11):1875-1881
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the method of chemical pattern recognition for wild germplasm resource of Ophiopogon japonicus in Sichuan Province. Methods: To establish chromatographic fingerprint of germplasm resource of O. japonicus in Sichuan by HPLC, according to the HPLC fingerprint, 26 wild germplasm resources of O. japonicus from different habitats in Sichuan were comprehensively compared by SPSS 17.0 software with principal component analysis and cluster analysis; and the correctness of "principal component - cluster analysis" was varified by the software of similarity evaluation. Results: Four principal components were extracted from 26 samples of O. japonicus and the samples were divided into four major categories; Compared with the direct clustering according to common peaks, the results of "principal component - cluster analysis" were more closer to the truth of similarity evaluation. Conclusion: The method of chemical pattern recognition is used for "principal component - cluster analysis" according to HPLC fingerprint of O. japonicus in Sichuan by SPSS 17.0 software. Its operation is simple and convenient, the result of statistics is reliable and able to provide a reference for the chemometrics classification and quality evaluation of O. japonicus.
