Experimental study on gambogic acid-induced gastric cancer cell apoptosis and anti-tumor metastasis
- Author:
Kai-Fei HUANG
1
Author Information
1. Drug R and D Center
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Gambogic acid;
Invasion;
Metastasis;
Tumor
- From:
Chinese Traditional and Herbal Drugs
2010;41(11):1823-1828
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the inhibition of gambogic acid on proliferation and metastasis ability of BGC-803 cells and to study their preliminary mechanism further. Methods: Methyl thiazolyl tetrazolium (MTT) and Hoechst staining were used to study the effects of gambogic acid-induced apoptosis in gastric cancer cell line BGC-803; Invasive methods were used to study the effects of invasion, adhesion, migration by gambogic acid; The influence of gambogic acid on the expression of bcl-2, bax, and ICAM-1 gene in gastric cancer cells was evaluated by RT-PCR and Western-blotting. Results: Gambogic acid was found not only to inhibit the growth of BGC-803 cells but also to induce the cell apoptosis in a dose- and time-dependent manner. The ability of adhesion, migration, and invasiveness was clearly inhibited by gambogic acid. With the increase of gambogic acid concentration, the expression of bcl-2, ICAM-1 gene, and protein were down-regulated and the expression of bax gene and protein were up-regulated. Conclusion: Gambogic acid could inhibit the growth of gastric cancer cells BGC-803 significantly, have a significant role in promoting tumor cell apoptosis, and inhibit tumor cell metastasis-associated properties. The mechanism may be related to decrease of bcl-2, ICAM-1 expression and increase bax expression.
