Effects of triptolide on cell cycle and expression of P21wap1/cip1 and P27kip1 in human multiple myeloma RPMI 8226 cells
- Author:
Yuan LIU
1
Author Information
1. Department of Hematology
- Publication Type:Journal Article
- Keywords:
Cell cycle;
P21wap1/cip1;
P27kip1;
RPMI 8226 cells;
Triptolide
- From:
Chinese Traditional and Herbal Drugs
2010;41(11):1819-1823
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of triptolide on cell proliferation, cell cycle distribution, DNA and protein expression regulation of P21wap1/cip1 and P27kip1 in human multiple myeloma RPMI 8226 cells. Methods: Cell viability was detected by MTT assay and cell cycle distribution was measured by flow cytometry. Effect on mRNA expression of P21wap1/cip1 and P27kip1 was detected by RT-PCR. The change of protein expression was measured by Western blotting. Results: Triptolide of varying concentration significantly induced the inhibition of proliferation in a dose-dependent manner and G0/G 1 phase arrest of the cell cycle progression. The events were coincided with the upregulation of the mRNA and protein expression of P21wap1/cip1 and P27kip1. Conclusion: These results suggest that triptolide might exhibit its strong antitumor effect via alternation of P21wap1/cip1 and P27kip1. It provides framework for a clinical evaluation of triptolide.
