Inhibitory mechanism of oridonin on G2/M phase arrest in SGC-7901 cell growth
- Author:
Yu-Bin JI
1
Author Information
1. Life Sciences and Environmental Sciences Research Center
- Publication Type:Journal Article
- Keywords:
Cdk1;
Cell cycle;
CyclinB1;
Human stomach cancer SGC-7901 cells;
Oridonin
- From:
Chinese Traditional and Herbal Drugs
2010;41(12):2024-2026
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of oridonin on G2/M phase arrest of human stomach cancer SGC-7901 cell growth in vitro and its molecular mechanism. Methods: The inhibition of oridonin on SGC-7901 cells was detected by MTT assay. Effect of oridonin on the phase distribution of SGC-7901 cell cycle was observed by flow cytometry (FCM). Western blotting was used to analyze the expression of Cdk1 and CyclinB1. Results: IC50 of oridonin on SGC-7901 cells was 15. 64 μmol/L. The effect of oridonin on SGC-7901 cells presented the increasing percentages of cells in G2/M phase as the concentration of oridonin increased. With the increase of oridonin dose, the expression of Cdk1 and CyclinB1 proteins was remarkably decreased. Conclusion: Oridonin can down-regulate the expression of Cdk1 and CyclinB1, which may be the mechanism of arresting SGC-7901 cells in G2/M phase.
