Protection and mechanism of Di'ao Xinxue Kang against myocardial ischemia-reperfusion injury in rats
- Author:
Hong CHEN
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Di'ao Xinxue Kang (DAXXK);
Endothelial function;
Myocardial ischemia-reperfusion;
Oxidative injury
- From:
Chinese Traditional and Herbal Drugs
2010;41(12):2018-2023
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the cardioprotective effect of Di'ao Xinxue Kang (DAXXK) and to explore the mechanisms on myocardial ischemia-reperfusion (I/R) injury in rats. Methods: The myocardial ischemia reperfusion injury model was established by the ligation of left descending coronary artery for 30 min and reperfusion for 120 min in rats. By using it, the effects of DAXXK different dosage groups on the ST segment of ECG, onset and duration of ventricular arrhythmia (VA) , the score of arrhythmia, and the effects on myocardial infarction size and morphologic change after myocardial injury in rats were observed. Myocardial apoptosis was detected using TUNEL method. Expressions of apoptosis-associated protein Fas and FasL were measured using the immunohistochemical method. Total-superoxide dismutase (T-SOD) was detected by spectrophotometer. NO and ET-1 in serum were detected by ELISA. Mn-SOD mRNA was measured by RT-PCR method. Results: Compared with I/R group, DAXXK with dose-dependent brought down the ST segment, decreased score of arrhythmia, diminished myocardial infarction size and decreased myocardial swelling, interstitial hemorrhage, and inflammatory cell infiltration, as well as a markedly improved cardiac function. Compared with I/R group, apoptosis was decreased, positive indexes of Fas and FasL were lessened. The myocardial antioxidative activity after myocardial I/R injury was increased and vascular endothelial function was improved by DAXXK in a dose-dependent manner. Conclusion: DAXXK could protect myocardium against I/R injury. The protective mechanisms could involved in the myocardial antioxidative activity, antiapoptosis action, and improvement of vascular endothelial function.
