Identification of ginsenosides from Panax quinque folius in Xinyue Capsula by LC-ESI-MSn
- Author:
Lin YANG
1
Author Information
1. Xiyuan Hospital
- Publication Type:Journal Article
- Keywords:
Ginsenosides;
LC-ESI-MSn;
Xinyue capsula
- From:
Chinese Traditional and Herbal Drugs
2010;41(12):1942-1947
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an LC-MS method for identification of the main components in Xinyue Capsula. Methods The main components in Xinyue Capsula were detected with the LC-ESI-MS in negative monitoring mode. These components were further analyzed by LC-MS2iv and MS3 spectra, and by comparing to the corresponding standards and the references, these components were elucidated. Results: Eighteen major ginsenosides, which were belonging to protopanaxadiol, protopanaxatriol, and ocotillol type saponin, were found in Xinyue Capsula. With the corresponding reference substances, ten of the 18 components were domenstrated as ginsenoside Re, Rg1, pseudoginsenoside F11, ginsenoside Rg2, Rc, Rb 2, Rb3, Rd, F2, and 20(S)-ginsenoside Rg 3. Conclusion: The LC-MSn method established in the pre sent study could simultaneously analyze various kinds of ginsenosides, which include ocotillol, protopanaxadiol, and protopanaxtiol type ginsenoside. This method is sensitive, rapid, and simple for analyzing and identifying the ginsenosides in Xinyue Capsula and Panax quinque folius.
