Chemical constituents of anti-oxidative activities from seeds of Jatropha curcas
- Author:
Ling LI
1
Author Information
1. College of Chinese Materia Medica
- Publication Type:Journal Article
- Keywords:
Anti-oxidative avtivity;
Jatrophasin A;
The seeds of Jatropha curcas L.
- From:
Chinese Traditional and Herbal Drugs
2010;41(12):1932-1936
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the chemical constituents with anti-oxidative activities from the seeds of Jatropha curcas. Methods: DPPH Radical scavenging assay was used for screening the active fractions. Using activity-guided- isolation, the active fractions were separated by column chromatography with silica gel, ODS, and Sephadex LH-20. The isolated compounds were then identified by spectral analyses (UV, IR, ESI-MS, HR-ESI-MS, 1H-NMR, 13C-NMR, and 2D-NMR). Anti-oxidative activities of all the isolated compounds were determined finally. Results: The n-butanol layer of ethanol extract from the seeds of J. curcas showed stronger activity and was studied further. Six compounds were isolated from this active layer and their structures were identified as jatrophasin A (3, 4, 4′, 5′-tetrahydroxyl- 5′-methoxyl-bisepoxylignan, 1), (±)-3, 3′- bisdemethylpinoresinol (2), 7-epi-sesamin-dicatechol (3), isoprincepin (4), β-sitosterol (5), and β-daucosterol (6). Conclusion: Compounds 3 and 4 show the strong, 1 and 2 show the medium anti-oxidative activities. Compound 1 is a new compound, named jatrophasin A, compounds 2, 3, and 4 are reported for the first isolation from the plants of Jatropha L.
