Protection of Eucommia ulmoides polysaccharide on liver fibrosis
- Author:
Cheng-Yan ZHOU
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
Carbon tetrachlotide (CCl 4);
Eucommia ulmoides oliv. Polysaccharide (EUP);
Laminin (LN);
Liver fibrosis;
Transforming growth factor-β1 (TGF-β1)
- From:
Chinese Traditional and Herbal Drugs
2011;42(2):324-329
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the protective effect of Eucommia ulmoides polysaccharide (EUP) on liver-fibrosis rats and investigate its mechanism. Methods: Models of liver-fibrosis rats induced by carbon tetrachlotide (CCl 4) were established by sc injection of pure CCl4 (5 mL/kg) and then peanut oil with 40% CCl4 (3 mL/kg) to the back of rats for eight weeks. After the models were ig administrated by EUP for eight weeks, the indexes of the liver and spleen in liver-fibrosis rats were calculated; ALT and AST activities and contents of TP and ALB in serum were examined; Meanwhile, the ratio between ALB and GLOB (AJG) was computed; The four levels of HA, LN, PCIII, and IV-C in serum of liver-fibrosis rats were determined. SOD activities and Hyp, MDA, and GSH-Px levels in liver tissue were examined; The expression of transforming growth factor-β1 (TGF-β1) in liver was observed. Results: EUP could obviously be against the index increasing of the liver and spleen (P<0.01) in liver-fibrosis rats induced by CCl4; remarkably inhibit the increasing of ALT and AST activities in serum (P<0.01); decrease the content of HA, LN, PCIII, IV-C, and GLOB in serum (P<0.01); increase the content of TP and ALB, and ratio of A/G in serum (P<0.01); decrease the MDA amd Hyp levels in liver tissue (P<0.01); and improve SOD activities and GSH-Px levels in liver tissue (P<0.01); and decrease the expression of TGF-β1 as well. The best effect of EUP was observed in the high-dose group and inhibition of EUP on fibrosis was in a dose-dependent manner. Conclusion: EUP has the remarkable protective effects on liver-fibrosis rats.
