Effect of tetrandrine on cytokines in LPS-stimulated RAW264.7 cell
- Author:
Fu-Ling LUO
1
Author Information
1. Chongqing Medical University
- Publication Type:Journal Article
- Keywords:
IL-10;
Lipopolysacchride (LPS);
NF-κB;
Raw264.7 cells;
Tetrandrine (Tet);
TNF-α
- From:
Chinese Traditional and Herbal Drugs
2011;42(3):542-545
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of tetrandrine (Tet) on expressions of proinflammatory and anti-inflammatory cytokine in lipopolysaccharide(LPS)- stimulated RAW264.7 cells. Methods: RAW 264.7 cells were cultured in media containing 1 μg/mL LPS and different doses of Tet. The proliferation of cells was examined by MTT assay, and nuclear translocation of NF-κB was detected and analyzed by the laser scanning confocal microscope (LSCM), and the levels of IL-6, TNF-α, and IL-10 were tested by ELISA. Results: Compared with the control group, Tet had no influence on the proliferation of RAW264.7 cells no matter with LPS or not when its concentration lower than 1 μmol/L; But when its concentration higher than 10 μmol/L, Tet significantly inhibited the growth of the cells whether LPS existed or not. Meanwhile, Tet significantly decreased the levels of IL-6, and TNF-α induced by LPS, and upregulated IL-10 in supernatant. At the mean time, results from LSCM showed that in Tet 1 μmol/L group, positive cells (dyeing-red p65 of NF-κB was translocated into nucleuses and made the nuclueses red) decreased siglificantly, while negative cells (dyeing-red p65 of NF-κB was collectted in endochylema and nucleuses became vacant) were predominantly founded. Conclusion: Tet has anti-inflammatory effect, which might be mediated by downregulating inflammatory factors IL-6, TNF-α through NF-κB signal pathway as well as increasing levels of anti-inflammatory factor IL-10.
