Separation and preparation of alkaloids in Nelumbinis Folium by pH-zone-refining counter-current chromatography
- Author:
Zhen-Jia ZHENG
1
Author Information
1. College of Food Science and Engineering
- Publication Type:Journal Article
- Keywords:
Alkaloids;
N-nornuciferin;
Nelumbinis Folium;
Nuciferine;
O-nornuciferin;
pH-zone-refining counter-current chromatography (pH-ZRCCC);
Roemerine
- From:
Chinese Traditional and Herbal Drugs
2011;42(4):684-686
- CountryChina
- Language:Chinese
-
Abstract:
Objective: A novel method for the separation of alkaloids from Nelumbinis Folium by pH-zone-refining counter-current chromatography (pH-ZRCCC). Methods: The two-phase solvent system composed of MtBE-methanol-water (4:1:5), 10 mmol/L triethylamine (TEA) in organic stationary phase and 5 mmol/L HCl in aqueous mobile phase. The upper phase was used as the stationary phase, while the lower phase was used as the mobile phase. In one-step elution under the conditions of a flow rate of 1.5 mL/min, 800 r/min and the effluent was detected at 254 nm. Results: From 2.18 g of the crude extract, 110 mg N-nornuciferin, 420 mg O-nornuciferin, 310 mg nuciferine, and 190 mg roemerine were obtained and each with a purity of over 98% as determined by HPLC. The structures of the isolated compounds were identified by HPLC, MS, and 1H-NMR. Conclusion: The present pH-ZRCCC method may be applied to the purification of other various alkaloids from natural products.
