Effect of ginseng polysaccharide on gene expression profile of K562 cells
- Author:
Jian-Ping LI
1
Author Information
1. Department of Histology and Embryology
- Publication Type:Journal Article
- Keywords:
Antitumor;
Gene chip;
Gene expression profile;
Ginseng polysaccharide;
K562 cells
- From:
Chinese Traditional and Herbal Drugs
2011;42(5):940-943
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of ginseng polysaccharide on the gene expression profile of human erythroleukemia K562 cells cultured in vitro. Methods: The total RNAs treated with ginseng polysaccharide for 48 h were extracted from both K562 cells and the control K562 cells. All the RNAs were purified into mRNA and then convened to double-stranded cDNAs by reverse transcription. The cDNAs were labeled with a fluorescence dye Cy3 or Cy5 and hybridized to a Illumina whole-genome oligonucleotide microarray. The change in the gene expression profile of K562 cells following treatment with ginseng polysaccharide was analyzed by using bioinformatics. Results: A total of 306 differential genes were found. Of which 220 were up-regulated and 86 were down-regulated. Their biologic function was classified. Conclusion: Tumor cell differentiation is multiple genes comprehensive results, the screening of the genes may be meaningful for understanding tumorigenesis, tumor development, reverse polarization, and finding potential drug targets.
