Analysis of alkaloids in Nelumbo nucifera leaves by solid phase extraction-rapid resolution liquid chromatography-quadrupole-time of flight mass spectrometry
- Author:
Zhen-Jia ZHENG
1
Author Information
1. College of Food Science and Engineering
- Publication Type:Journal Article
- Keywords:
Alkaloids;
Cation exchange;
Nelumbo nucifera Gaertn. leaves;
Rapid resolution liquid chromatography- quadrupole-time of flight (RRLC-Q-TOF) mass spectrometry;
Solid phase extraction (SPE)
- From:
Chinese Traditional and Herbal Drugs
2011;42(6):1066-1068
- CountryChina
- Language:Chinese
-
Abstract:
Objective: A novel method for the analysis of alkaloids in Nelumbo nucifera leaves was established by SPE-RRLC-Q-TOF mass spectrometry. Methods: The crude sample was extracted by 1% HCl with ultrasound-assisted extraction, then purified by SPE column, and eluted with ammonia-methanol (5:95). After concentration, the residue was dissolved by methanol solution. The real sample was analyzed by RRLC-Q-TOF. A Welch Materials C18 column was applied in the RRLC separation using acetonitrile and water as mobile phase. The elutes were detected by Q-TOF to obtain the MS spectra with extract molecular weights under positive ion mode. Results: Nine alkaloids were identified. Conclusion: This method can be used to rapidly determine the alkaloids of N. nucifera leaves.
