Growth characteristics of Epimediun wushanense in cell suspension culture
- Author:
Su-Ju HAN
1
Author Information
1. Mianyang Normal University
- Publication Type:Journal Article
- Keywords:
Epimediun wushanense T. S. Ying;
Flavonoids;
Growth characteristics;
Growth curve;
Suspension culture
- From:
Chinese Traditional and Herbal Drugs
2011;42(8):1609-1613
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To provide biotechnology reference for the utilization of Epimedium wushanense. Methods: Some subculture conditions were determined and the growth characteristics of suspension cells were observed every 3 d. Results: The cell showed S-shaped growth curve and the content of nitrate, ammonia salt, phosphate, and sucrose all showed sharply decreasing trend during the initial cell suspension culture of E. wushanense. Then the respective changes slowed down. The optimum illumination duration was 12 h. The highest output of flavonoids was 27.854 82 mg/L under 12 h illumination. Conclusion: The growth characteristics research lays the foundation of mass natural active ingredient in cell suspension culture production of E. wushanense.
