Optimization of synthetic process of PEGylated puerarin by orthogonal test with multi-index
- Author:
Xin-Yi LIU
1
Author Information
1. Jiangsu Center for Drug Screening
- Publication Type:Journal Article
- Keywords:
HPLC;
Multi-index evaluation;
Orthogonal test;
PEGylated puerarin (PEG-PUE);
Puerarin (PUE)
- From:
Chinese Traditional and Herbal Drugs
2011;42(8):1528-1531
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize the synthesis of PEGylated puerarin (PEG-PUE) by orthogonal test with multi-index evaluation. Methods: The research has focused on the optimized interacting factors of the synthetic process of PEG-PUE by HPLC, such as ratio of PEG and PUE, catalyst DMAP dosage, and reaction time, using the purity, drug loading, and yield of PEG-PUE as evaluation indexes. Results: The optimum synthesis conditions of PEG-PUE were as follows, PEG-EDC-PUE-DMAP (1 : 1.2 : 1.2 : 0.3) and reaction time 12 h. Conclusion: The optimum synthesis condition of PEG-PUE is reasonable, as well as feasible.
