Effects of growth regulator and culture methods on rooting of Ajuga lobata and content of β-ecdysone
- Author:
Xiao-Jie ZHAO
1
Author Information
1. College of Life Science
- Publication Type:Journal Article
- Keywords:
β-ecdysone;
Ajuga lobata D. Don;
Indole butyric acid (IBA);
MS medium;
NAA
- From:
Chinese Traditional and Herbal Drugs
2011;42(9):1828-1832
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of indole butyric acid (IBA) and NAA at different concentrations and culture methods on the growth of Ajuga lobata root and content of β-ecdysone. Methods: Taking MS as basic medium supplemented with IBA (0.5, 1.0, and 2.0 mg/L) or NAA (0.3, 0.6, and 0.9 mg/L), the tissue culture was carried out. HPLC was used to determinate the content of β-ecdysterone. Results: The results showed that the roots could grew well on MS as basic medium supplemented with IBA 0.5-1 mg/L. The best culture condition for the highest β-ecdysone accumulation was MS medium supplemented with IBA 2 mg/L. The content of β-ecdysterone in the whole plant and the roots of A. lobata with tissue culture was twice times of hydroponics and about three times of cultivation. Conclusion: MS medium supplemented with IBA at the proper concentration could promote the root growth of A. lobata. The content of β-ecdysterone in the root of A. lobata is the highest in tissue cultures.
