Analysis of genetic relationship and quality assessment on plants in Flemingia Roxb. ex Ait. et Ait. f.
- Author:
Zhong-Lian ZHANG
1
Author Information
1. Yunnan Branch of Institute of Medicinal Plant Development
- Publication Type:Journal Article
- Keywords:
Flemingia Roxb. ex Ait. et Ait. f.;
Flemingiawallichii Wight et Am;
Genetic diversity;
Inter-simple sequence repeats (ISSR);
Total flavones
- From:
Chinese Traditional and Herbal Drugs
2011;42(9):1817-1821
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the genetic relationship of the plants in Flemingia Roxb. ex Ait. et Ait. f. and make preliminary quality accessment, in order to provide the theoretical basis for screening the new medicinal resource. Methods: Inter-simple sequence repeats (ISSR) markers were used to analyze the genetic relationship of 14 species in Flemingia Roxb. ex Ait. et Ait. f.. Meanwhile, spectrophotometry was used to measure total flavones. Results: Thirty primers were screened out of 60 ISSR random primers and produced 367 bands totally. Among them 363 bands (98.91%) were polymorphic, four bands were owned by all; The genetic similarity (GS) was 0.5668-0.8338, showing abundant genetic diversity; F. wallichii and F. philippinensis have the closest genetic relationship, F. lineate and F. chappar have the largest genetic distance; The highest content of total flavones is in F. mengpengensis and secondary is in F. philippinensis, total content of flavones in F. wallichii is above average. Conclusion: The plants in Flemingia Roxb. ex Ait. et Ait. f. have the abundant genetic diversity. As medicinal plants, F. wallichii has the potential for replacing F. philippinensis.
