Interaction between curcumin and bovine serum albumin by fluorescence
- Author:
Rui-Jia LAN
1
Author Information
1. Faculty of Chemistry and Material Science
- Publication Type:Journal Article
- Keywords:
Binding reaction;
Bovine serum albumins (BSA);
Curcumin (CU);
Fluorescence quenching;
Hydrophobic force
- From:
Chinese Traditional and Herbal Drugs
2011;42(9):1715-1718
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the mechanism of interation between curcumin (CU) and bovine serum albumin (BSA). Methods: The style of quenching was investigated by changing the reaction temperature. The number and constant of binding sites of CU to BSA were calculated. Results: Static quenching was the main reason that caused the quenching of BSA fluorescence. The apparent combining constants (K0) were 5.26 × 106 at 21°C and 4.60 × 106 at 31°C, and the number of binding sites (n) was 1.35. By calculating the thermodynamic parameters, it can be deduced that hydrophobic force is the main binding force between CU and BSA. Conclusion: Fluorometric analysis is a good method for the study on CU-BSA binding reaction. It has the advantages of high speed and high sensitivity.
