Establishment of "combinatorial screening" library of efficacy substances for Qingluo Tongbi Decoction
- Author:
Xin-Long ZHANG
1
Author Information
1. Key Laboratory of Separation Engineering for Chinese Medicine Compound
- Publication Type:Journal Article
- Keywords:
Building block;
Combination;
Efficacy substances;
Qingluo tongbi decoction (qtd);
Screening
- From:
Chinese Traditional and Herbal Drugs
2012;43(1):60-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective Attempting to create a chemical diversity" combinatorial library consisting of active components of Chinese materia medica compounds. Methods This paper was based on the theory of "thinking and method of 'combinatorial screening' of Chinese material medica compound", using the Qingluo Tongbi Decoction (QTD) which has exact effect against rheumatoid arthritis as system. Re-combination group of the formulation was used and various industrial separation techniques, such as resin and membrane, were used to replace the diversity of combinatorial chemistry synthetic route. Different products were used to establish combinatorial library using "Building Block". Results Different contents of active components in each "Building Block" were found during the establishment of combinatorial libraries. AB-8 resin had good adsorption and desorption functions on sinomenine, and good refined effects on saponin as well. The contents of kirenol were different in each "Building Block" of different combinations. Each "Building Block" had a relative high content of total flavonoids. Conclusion Different combinations and separation techniques lead the differences in types and contents of chemical constituents. So certain pharmacodynamic experiments are needed to evaluate the preparation and to filter out the relevant active "Building Blocks".
