Genomic DNA cloning and analysis of uridine diphosphate-glucosyltransferase gene from Rhodiola crenulata
10.7501/j.issn.0253-2670.2013.06.020
- Author:
Kai CHANG
1
Author Information
1. Chongqing Engineering and Technology Research Center for Sweetpotato
- Publication Type:Journal Article
- Keywords:
Genome;
Promoter;
Rhodiola crenulata (Hook. F. et Thoms.) S. H. Fu;
Salidroside;
Uridine diphosphate-glucosyltransferase
- From:
Chinese Traditional and Herbal Drugs
2013;44(6):736-743
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To obtain the genomic DNA sequence of uridine diphosphate (UDP)-glucosyltransferase (GT) gene from Rhodiola crenulata and to analyze its DNA sequence at the level of bioinformatics. Methods: The optimized CTAB method was used to extract the genomic DNA from R. crenulata, and after three times genomic walking, the genomic DNA sequence of UDPGT in R. crenulata (RcUDPGT) was obtained by hiTAIR-PCR. The DNA sequence was analyzed by bioinformatics method. Results: The 2 977 bp DNA sequence of RcUDPGT was obtained, which contained the 1 499 bp gene sequence (including the 82 bp intron sequence) and 1 500 bp 5' upstream flanking sequence of coding region (including promoter sequence). The bioinformatic analysis showed that the RcUDPGT was hydrophilic, located in the cytoplasm, and had high homology with UDPGTs of other plants. The tertiary structure of RcUDPGT indicated that protein had UDPGT functional sites. Promoter analysis showed that it contained cis-acting elements responding to light, heat, pressure, temperature, anaerobic, anthocyanins biosynthesis sequences, etc. Conclusion The structure of RcUDPGT gene is integral and has functional sites. The research provides the reference for the molecular biology and metabolic engineering of R. crenulata.
