Glutamate dehydrogenase antigen detection in Plasmodium falciparum infections.
10.3347/kjp.1996.34.4.239
- Author:
Neira de DOMINIGUEZ
1
;
Alexis RODRIGUEZ-ACOSTA
Author Information
1. Universidad Central de Venezuela Tropical Medicine Institute Immunochemistry Department Apartado 47423
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- MeSH:
parasitology-protozoa;
antigen;
enzymes;
glutamate dehydrogenase;
malaria;
diagnosis;
Plasmodium berghei;
Plasmodium cathemerium;
Plasmodium falciparum;
Plasmodium vivax;
enzyme-linked immunosorbent assay
- From:The Korean Journal of Parasitology
1996;34(4):239-246
- CountryRepublic of Korea
- Language:English
-
Abstract:
The usefulness of malaria diagnosis by Plasmodium falciparum-GDH (NADP+), obtained by affinity chromatography, is demonstrated in ELISA assays, testing IgG antibodies against GDH (NADP+) from patients with acute malaria, who have had two or more episodes of malaria, or from sera of hyperimmune patients. GDH (NADP+) thermal stability was demonstrated in a high heat resistance assay. The immunofluorescence assay demonstrated that anti-culture (P. falciparum) supernatant serum and anti-GDH (NADP+) of Proteus spp, recognized epitopes in Venezuelan isolates, and Colombian and Brasilian malarial strains. The antigen is soluble, with high specificity, is a potent immunogen and is thermoresistant.
