Formula optimization and preparation technology of glycyrrhetinic acid- tanshinone IIA compound liposomes
10.7501/j.issn.0253-2670.2013.07.008
- Author:
Jia-Hao LIN
1
Author Information
1. College of Chinese Material Medical
- Publication Type:Journal Article
- Keywords:
Compound liposomes;
Film dispersion ultrasonic probe method;
Glycyrrhetinic acid;
Low-speed centrifugation;
Tanshinone IIA
- From:
Chinese Traditional and Herbal Drugs
2013;44(7):816-819
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the preparation technology of glycyrrhetinic acid (GA)-tanshinone IIA (Tan IIA) compound liposomes (GT-Lip). Methods: The compound liposomes were made of soybean phosphatidylcholine (SPC) and cholesterol (Ch) by film dispersion ultrasonic probe method. Hydration temperature and ultrasonic power were optimized by single factor tests and the optimum formulation was selected by orthogonal design. The encapsulation efficiencies (EE) of GA and Tan IIA were determined by low-speed centrifugation. The particle size and Zeta potential of liposomes were detected by dynamic light scattering particle size meter. Transmission electron microscopy was used to observe morphous. Results: The optimal preparation conditions were as follows weight ratio of SPC-Ch 6:1, molar ratio of SPC-Tan IIA and SPC-GA was 30:1 and 24:1, hydration temperature 30 °C, and ultrasonic power 380 W for 5 min. Using the optimal method, the EE values of GA and Tan IIA in GT-Lip were (81.50 ± 0.76)% and (98.63 ± 0.90)% (n = 3), and the average particle size of liposomes was (120.5 ± 1.62) nm and the Zeta potential of liposomes was (-19.00 ± 0.98) mV (n = 3). Conclusion: The optimal preparation method of GT-Lip in this study is stable and feasible.
