Molecular authentication and phylogenetic relationship of Perilla frutescens and its varieties
10.7501/j.issn.0253-2670.2013.08.021
- Author:
Zhi XIA
1
Author Information
1. Agronomy College of Henan Agricultural University
- Publication Type:Journal Article
- Keywords:
Authentication;
ITS;
Perilla frutescens (Linn.) Britt.;
Perilla L.;
PsbA-trnH
- From:
Chinese Traditional and Herbal Drugs
2013;44(8):1027-1032
- CountryChina
- Language:Chinese
-
Abstract:
Objective: In order to investigate the phylogenetic relationship of Perilla frutescens and its varieties, and to identify P. frutescens and its varieties in Perilla L. Methods: Total genomic DNA was isolated from P. frutescens and its varieties. Nuclear DNA ITS and chloroplast gene psbA-trnH sequence were amplified and sequenced. The inter- and intra-specific Kimura 2-Parameter (K2P) distances were calculated. Phylogenetic analyses including Maximum Parsimony (MP) and Neighbor-Joining (NJ) of nuclear DNA (ITS) and chloroplast gene psbA-trnH sequences were conducted individually. Results: The MP tree (ITS and psbA-trnH) indicated that the different geographical population of P. frutescens and P. frutescens var. arguta formed a monophyletic clade [bootstrap (BS) = 100% and 93%], which was a sister branch to P. frutescens var. crispa (BS = 95% and 90%). Wild P. frutescens var. purpurascens and P. frutescens var. auriculato-dentata formed a monophyletic clade (BS = 100% and 90%). The inter-specific genetic distances (ITS and psbA-trnH) of P. frutescens, P. frutescens var. arguta, and other varieties were 0.008 21-0.018 18 and 0.002 38-0.029 31, which were obviously higher than those of P. frutescens and P. frutescens var. arguta at 0-0.001 65 and 0, and were higher than intra-specific genetic distances of each other variety. Conclusion: P. frutescens and P. frutescens var. arguta should be classified into one species. P. frutescens is relatively closed to P. frutescens var. crispa. The closest relationship is found between wild P. frutescens var. purpurascens and P. frutescens var. auriculato-dentata. ITS and psbA-trnH are two efficient barcodes for the identification of P. frutescens and its varieties.
