Early intervention of Didang Decoction on macroangiopathy in type 2 diabetic rats and its mechanisms
10.7501/j.issn.0253-2670.2013.08.018
- Author:
Cong-Qing PAN
1
Author Information
1. Tianjin Medical University Metabolic Diseases Hospital
- Publication Type:Journal Article
- Keywords:
Didang Decoction;
Intercellular cell adhesion molecule-1;
Macroangiopathy;
Matrix metalloproteinases-9;
NF-κB;
Type 2 diabetes;
Vascular cell adhesion molecule-1
- From:
Chinese Traditional and Herbal Drugs
2013;44(8):1013-1016
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effects of the early intervention with Didang Decoction (DDD) on macroangiopathy in type 2 diabetic rats. Methods: The type 2 diabetic rat model was established using high-fat diet and Streptozotocin (STZ), and the rats were divided into control, model, Pioglitazone (2.7 mg/kg), Simvastatin (1.8 mg/kg), early-, mid-, and late-term DDD-intervene groups (ig administered with 3.24 g/kg DDD once daily, before 4 weeks, at the same time, and after 4 weeks of STZ administration, respectively), the rats in each group were administered until 24 weeks of STZ administration. The immunohistochemical and pathological changes of intercellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in the aorta were observed, and the expression of nuclear factor-kappa B (NF-κB) and matrix metalloproteinases-9 (MMP-9) in aorta was detected using Western blotting. Results: Compared with the model group, the ICAM-1 expression decreased in early- and mid-term DDD-intervene groups, and Simvastatin group (P < 0.05), the VCAM-1 expression decreased in early-term DDD-intervene and Simvastatin groups (P < 0.05), the protein expression of NF-κB and MMP-9 was lower in early- and mid-term DDD-intervene groups (P < 0.05), and it is the most obvious in early-term DDD-intervene group. Conclusion: The contents of ICAM-1 and VCAM-1 as well as the protein expression of NF-κB and MMP-9 in type 2 diabetic rats decrease after early-term DDD-intervene, which could regulate NF-κB signaling pathway to delay the development of diabetic macroangiopathy.
