Simultaneous determination of 10 constituents in Shuangqing Yanhou Tablets by HPLC-QAMS
10.7501/j.issn.0253-2670.2013.08.011
- Author:
Bing HE
1
Author Information
1. Research Center for Drug
- Publication Type:Journal Article
- Keywords:
Ammonium glycyrrhizinate;
Artemisia ketone;
Chlorogenic acid;
Citric acid;
Gallic acid;
HPLC;
Quantitative analysis on multi-components by single marker;
Relative correction factor;
Shuangqing Yanhou Tablets
- From:
Chinese Traditional and Herbal Drugs
2013;44(8):974-981
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a new method for quantitative analysis on multi-components by single marker (QAMS) and validate its feasibility and technical adaptability in analysis on Shuangqing Yanhou Tablets (SYTs) for the simultaneous determination of 10 main constituents (citric acid, gallic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, ammonium glycyrrhizinate, and artemisia ketone). Methods: Using SYTs as objects and the above 10 constituents as indexes, three correction methods were used to establish the relative correction factor (fk/s) between each component and gallic acid, respectively then to calculate the amount of each component and finally to achieve QAMS. At the same time, the external reference method and regression equation method were used to determine the amounts of the above 10 constituents, to compare the difference between the calculated and real data of the three fk/s, and to validate the correctness and adaptability of QAMS. Results: No significant difference was found in the quantitative results of 10 active constituents in three batches of SYTs determined by the calculated and real data. Conclusion: The QAMS by three correction methods is feasible and accurate to evaluate the contents of the 10 constituents in SYTs.
