Analysis on chemical constituents in ethanol extract from vanilla pod in various habitats and quantitative determination of vanillin by GC-MS
10.7501/j.issn.0253-2670.2013.08.007
- Author:
Hao SUN
1
Author Information
1. Pharmacy College
- Publication Type:Journal Article
- Keywords:
Ethanol extract;
GC-MS;
Quantitative determination;
Vanilla pod;
Vanillin
- From:
Chinese Traditional and Herbal Drugs
2013;44(8):955-959
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the chemical constituents in ethanol extracts from vanilla pod in Hainan (China) and Papua-New Guinea and to assay the content of vanillin. Methods: Comparing various pretreatment methods, GC-MS analysis method was selected and the column was packed with Agilent DB-5MS capillary column (30 m × 0.32 mm, 0.25 μm); column temperature: the column initial temperature was 60 °C, kept for 1 min, rose to 260 °C at the rate of 10 °C/min, kept for 6 min, rose to 290 °C at the rate of 6 °C/min, kept for 8 min; carrier gas: helium for comparing the difference of the constituents from the above two habitats. Results: Because of the different habitats, 34 and 32 compounds were respectively identified from vanilla pod and 18 compounds were common components. Vanillin had a high content in vanilla pod and presented a good linearity with the peak area at the concentration of 0.04-1.10 mg/mL (r = 0.999 8, n = 5). The average recovery was 100.05% with the RSD of 1.88%. Conclusion: GC-MS is quick, simple, convenient, and effective to analyze the constituents in the ethanol extracts from vanilla pod, and it is also reliable and reproducible to determine the content of vanillin.
