Effect of crocin-1 on learning, memory, and expression of SIRT1 in hippocampus of rats under acute hypoxia
10.7501/j.issn.0253-2670.2013.10.021
- Author:
Xiao-Yan ZHANG
1
Author Information
1. Department of Basic Medicine
- Publication Type:Journal Article
- Keywords:
Acute hypoxia;
Crocin-1;
Hippocampus;
Learning and memory;
Silent information regulator l
- From:
Chinese Traditional and Herbal Drugs
2013;44(10):1314-1317
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of crocin-1 on behavior of learning and memory in rats under acute hypoxia and on the expression of silent information regulator l (SIRT1) in hippocampus of rats. Methods: SD rats were randomly divided into five groups: control, hypoxia model, low-, mid-, and high-dose (25, 50, and 100 mg/kg) crocin-1 groups. The SD rats in crocin-1 groups were im administered once daily for 3 d and then were stimulated for 72 h under hypoxia. Morris water maze was used to investigate the learning and memory behaviors. Immunohistochemical staining and Western blotting were used to detect the SIRT1 expression. Results: The escape latency in crocin-1 groups was shorter than that in hypoxia model group, while the frequency of bestriding platform was increased (P < 0.05). Immunohistochemical staining and Western blotting both showed that the expression level of SIRT1 was higher in crocin-1 groups than that in hypoxia model group (P < 0.05), and the expression levels in mid- and high-dose groups were obviously higher. Conclusion: Crocin-1 could increase the expression of SIRT1 in hippocampus of rats, which maybe one of the important mechanisms for crocin-1 improving learning and memory function of SD rats under acute hypoxia.
