Extracting technology of guanfubase A from Aconitum coreanum by high-voltage pulsed electric field method
10.7501/j.issn.0253-2670.2013.10.011
- Author:
Yong-Zhi BAI
1
Author Information
1. Institute of Special Wild Economic Animal and Plant Science
- Publication Type:Journal Article
- Keywords:
Aconitum coreanum (LéVl.) Raipaics;
Extract yield;
Guanfubase A;
High-voltage plused electric field;
Orthogonal test
- From:
Chinese Traditional and Herbal Drugs
2013;44(10):1267-1270
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize the extracting technology for guanfubase A from Aconitum coreanum by high-voltage pulsed electric field method and to provide the reliable experimental data and theoretical basis for the industrial production. Methods: The content of guanfubase A was detected by HPLC in orthogonal test. Results: The optimal formulation was as follows: high voltage of 20 kV/cm, impulsion frequency of 10, and solid-to-solvent ratio of 1:14, with 90% ethanol as extracting solvent, and the influence factor in size was voltage, impulsion frequency, and solid-to-solvent ratio. The guanfubase A content and extract yield were up to 0.391% and 39.92% in A. coreanum, respectively. Conclusion: The extracting technology is feasible and economic, which has the advantage of time-saving and high recovery rate.
